Journal: Oncology Letters

Article Title: Role of non-coding RNAs in O 6 -methylguanine-DNA methyltransferase-positive glioblastoma (Review)

doi: 10.3892/ol.2026.15550

Figure Lengend Snippet: Core competing endogenous RNA network of miRNA, lncRNA and circRNA in regulating MGMT expression. Arrows denote promotion or positive regulation, while blunt-ended arrows represent inhibition or negative regulation. miRNA/miR, microRNA; lncRNA, long non-coding RNA; circRNA/circ, circular RNA; MGMT, O 6 -methylguanine-DNAmethyltransferase; Sp1, specificity protein 1; HOTAIR, HOX transcript antisense RNA; UCA1, urothelial carcinoma-associated 1; WDR62, WD repeat domain 62.

Article Snippet: Exosome-derived lncRNA HOTAIR from serum has been reported to promote TMZ resistance in GBM cells, whereas knockdown of lncRNA HOTAIR enhances TMZ sensitivity both in vivo and in vitro ( ).

Techniques: Expressing, Inhibition

Journal: Oncology Letters

Article Title: Role of non-coding RNAs in O 6 -methylguanine-DNA methyltransferase-positive glioblastoma (Review)

doi: 10.3892/ol.2026.15550

Figure Lengend Snippet: Schematic diagram illustrating the mechanisms by which lncRNAs TALC and PRADX regulate MGMT expression. Arrows denote promotion or positive regulation, while blunt-ended arrows represent inhibition or negative regulation. lncRNA, long non-coding RNA; MGMT, O 6 -methylguanine-DNA methyltransferase; PRADX, PRC2 and DDX5-associated lncRNA; TALC, temozolomide-associated lncRNA in glioblastoma recurrence; ATF3, activating transcription factor 3; P, phosphorylation; H3K27me3, trimethylation of histone H3 lysine 27; HDACI, histone deacetylase inhibitor; miR, microRNA.

Article Snippet: Exosome-derived lncRNA HOTAIR from serum has been reported to promote TMZ resistance in GBM cells, whereas knockdown of lncRNA HOTAIR enhances TMZ sensitivity both in vivo and in vitro ( ).

Techniques: Expressing, Inhibition, Phospho-proteomics, Histone Deacetylase Assay

Journal: Oncology Letters

Article Title: Role of non-coding RNAs in O 6 -methylguanine-DNA methyltransferase-positive glioblastoma (Review)

doi: 10.3892/ol.2026.15550

Figure Lengend Snippet: Core competing endogenous RNA network of miRNA, lncRNA and circRNA in regulating MGMT expression. Arrows denote promotion or positive regulation, while blunt-ended arrows represent inhibition or negative regulation. miRNA/miR, microRNA; lncRNA, long non-coding RNA; circRNA/circ, circular RNA; MGMT, O 6 -methylguanine-DNAmethyltransferase; Sp1, specificity protein 1; HOTAIR, HOX transcript antisense RNA; UCA1, urothelial carcinoma-associated 1; WDR62, WD repeat domain 62.

Article Snippet: Exosome-derived lncRNA HOTAIR from serum has been reported to promote TMZ resistance in GBM cells, whereas knockdown of lncRNA HOTAIR enhances TMZ sensitivity both in vivo and in vitro ( ).

Techniques: Expressing, Inhibition

Journal: Oncology Letters

Article Title: Role of non-coding RNAs in O 6 -methylguanine-DNA methyltransferase-positive glioblastoma (Review)

doi: 10.3892/ol.2026.15550

Figure Lengend Snippet: Schematic diagram illustrating the mechanisms by which lncRNAs TALC and PRADX regulate MGMT expression. Arrows denote promotion or positive regulation, while blunt-ended arrows represent inhibition or negative regulation. lncRNA, long non-coding RNA; MGMT, O 6 -methylguanine-DNA methyltransferase; PRADX, PRC2 and DDX5-associated lncRNA; TALC, temozolomide-associated lncRNA in glioblastoma recurrence; ATF3, activating transcription factor 3; P, phosphorylation; H3K27me3, trimethylation of histone H3 lysine 27; HDACI, histone deacetylase inhibitor; miR, microRNA.

Article Snippet: Exosome-derived lncRNA HOTAIR from serum has been reported to promote TMZ resistance in GBM cells, whereas knockdown of lncRNA HOTAIR enhances TMZ sensitivity both in vivo and in vitro ( ).

Techniques: Expressing, Inhibition, Phospho-proteomics, Histone Deacetylase Assay

Journal: Nature Communications

Article Title: The history and function of a circular RNA

doi: 10.1038/s41467-026-71822-0

Figure Lengend Snippet: A Schematic representation of the genomic locus of lncRNA Cyrano/OIP5-AS1. Exon 3 contains a single miR-7 perfect complementarity site (pink), which triggers miR-7 TDMD. Additionally, one of the several Cyrano conserved regions, a 300nt sequence (purple) flanking the miR-7 biding site emerged in vertebrates from Xenopus to Human, but is not present in zebrafish . B Evolutionary conservation of lncRNA Cyrano/OIP5-AS1. Phylogeny of several animal groups reflecting the expression of Cyrano/OIP5-AS1 from zebrafish to human. Conservation of 300nt sequence (purple) containing miR-7 biding site (red). C Relative RNA expression of lncRNA Cyrano/OIP5-AS1 across cells and tissues. Indicated is the relative expression of Cyrano across samples from high (purple) to low (gray) Left: brain region specific (cortex, hippocampus, hypothalamus, pituitary gland; Allen Brain Atlas). Middle: neuronal-type specific (inhibitory, excitatory, granular). D Normalized abundance of the Cyrano sequences across matched human and mouse organ systems (nervous, blood/immune, respiratory, digestive, urinary and skeletal). Expression was estimated from raw reads by counting exact k-mer matches to the probe sequences in each single-cell or spatial dataset (Rajewsky lab, unpublished sequence-search index). Values are scaled to the total number of informative k-mers per cell and sample. Expression units are species-normalized pseudocounts (scaled per 10⁶ k-mers per cell). Within the nervous system, the same approach resolves region- and cell-type–specific patterns for neuronal and glial subclasses of human and mouse . Datasets: Human Cell Atlas single-cell collections released up to 2024; Human Adult Brain Cell Atlas ; Mouse Brain Cell Atlas ; Tabula Muris (Tabula Muris Consortium 2018, 2020). Created in BioRender. https://BioRender.com/3it2qak .

Article Snippet: CDR1as is further embedded in a regulatory network with the lncRNA Cyrano.

Techniques: Sequencing, Expressing, RNA Expression, Single Cell

Journal: Nature Communications

Article Title: The history and function of a circular RNA

doi: 10.1038/s41467-026-71822-0

Figure Lengend Snippet: A Schematic representation of the dynamic molecular regulatory network involving Cdr1as/Cirs-7, miR-7, Cyrano, and miR-671 in neurons. Cdr1as directly interacts with miR-7 molecules , . Complete KO of Cdr1as/Cirs-7 destabilized miR-7 post-transcriptionally . Direct or indirect upregulation of miR-7 downregulates expression of Cdr1as , , . lncRNA Cyrano triggers degradation miR-7 molecules through a TDMD mechanism . KO of miR-7 site on Cyrano upregulates miR-7 expression . miR-671 destabilized Cdr1as/Cirs-7 by slicing of the circRNA molecule due to high complementarity biding , . Disruption of miR-671 binding site on Cdr1as/Cirs-7 increase the circRNA and miR-671 . Complete KO of Cdr1as/Cirs-7 upregulates miR-671 post-transcriptionally . Created in BioRender. https://BioRender.com/d639swi . B Schematic representation of in vitro stressors in forebrain neurons and their proven molecular effects on the network (Cdr1as/Cirs-7, miR-7, Cyrano). Purple: global depolarization by extracellular potassium . Blue: oxygen and glucose deprivation . Yellow: induction of homeostatic plasticity by Bicuculline . Created in BioRender. https://BioRender.com/xwkh5rs . C Schematic representation of permanent (pMCAo) and transient (tMCAo) ischemic stroke surgery in mouse brain. Resulting molecular effect on the network (Cdr1as/Cirs-7, miR-7, Cyrano), ischemic lesion size and behavioral changes. In the schematic the ischemic damage in pink and dotted line is representative of the relative lesion size as measured by MRI . Created in BioRender. https://BioRender.com/ijyhduh . D Schematic representation of neuronal network activity and glutamate secretion changes upon stress/stimulation in WT and Cdr1as-KO neurons. Left/Gray: physiological glutamate secretion in WT neurons and subsequent synchronous network activity. Middle/Orange: increased glutamate release and asynchronous network activity in Cdr1as-KO neurons . Right/Green: rescue of glutamate secretion and restored network activity in Cdr1as-KO neurons upon miR-7 upregulation after glutamatergic or electrical stimulation . Created in BioRender. https://BioRender.com/jpctgbe .

Article Snippet: CDR1as is further embedded in a regulatory network with the lncRNA Cyrano.

Techniques: Expressing, Disruption, Binding Assay, In Vitro, Activity Assay

Journal: Nature Communications

Article Title: The history and function of a circular RNA

doi: 10.1038/s41467-026-71822-0

Figure Lengend Snippet: A Schematic representation of the genomic locus of lncRNA Cyrano/OIP5-AS1. Exon 3 contains a single miR-7 perfect complementarity site (pink), which triggers miR-7 TDMD. Additionally, one of the several Cyrano conserved regions, a 300nt sequence (purple) flanking the miR-7 biding site emerged in vertebrates from Xenopus to Human, but is not present in zebrafish . B Evolutionary conservation of lncRNA Cyrano/OIP5-AS1. Phylogeny of several animal groups reflecting the expression of Cyrano/OIP5-AS1 from zebrafish to human. Conservation of 300nt sequence (purple) containing miR-7 biding site (red). C Relative RNA expression of lncRNA Cyrano/OIP5-AS1 across cells and tissues. Indicated is the relative expression of Cyrano across samples from high (purple) to low (gray) Left: brain region specific (cortex, hippocampus, hypothalamus, pituitary gland; Allen Brain Atlas). Middle: neuronal-type specific (inhibitory, excitatory, granular). D Normalized abundance of the Cyrano sequences across matched human and mouse organ systems (nervous, blood/immune, respiratory, digestive, urinary and skeletal). Expression was estimated from raw reads by counting exact k-mer matches to the probe sequences in each single-cell or spatial dataset (Rajewsky lab, unpublished sequence-search index). Values are scaled to the total number of informative k-mers per cell and sample. Expression units are species-normalized pseudocounts (scaled per 10⁶ k-mers per cell). Within the nervous system, the same approach resolves region- and cell-type–specific patterns for neuronal and glial subclasses of human and mouse . Datasets: Human Cell Atlas single-cell collections released up to 2024; Human Adult Brain Cell Atlas ; Mouse Brain Cell Atlas ; Tabula Muris (Tabula Muris Consortium 2018, 2020). Created in BioRender. https://BioRender.com/3it2qak .

Article Snippet: B Evolutionary conservation of lncRNA Cyrano/OIP5-AS1.

Techniques: Sequencing, Expressing, RNA Expression, Single Cell